Text Box: THIN-LAYER CHROMATOGRAPHY WITH DIRECT MASS SPECTROMETRIC ANALYTE DETECTION BEATE FUCHS AND JÜRGEN SCHILLER UNIVERSITY OF LEIPZIG, MEDICAL DEPARTMENT, INSTITUTE OF MEDICAL PHYSICS & BIOPHYSICS, HÄRTELSTR. 16/18, D-04107 LEIPZIG, GERMANY Abstract Thin-layer chromatography (TLC), and particularly high-performance thin-layer chromatography (HPTLC), is a widely used, fast, and inexpensive separation method. Even minor analytes of complex mixtures often can be separated from bulk constituents and monitored with high sensitivity and resolution. However, unambiguous assignment of the obtained spots to defined compounds often is difficult, because the application of common TLC staining methods reveals a substance class but not a specific compound. In the case of phospholipids, the lipid class, but not the detailed fatty acyl composition, can be determined. Combining TLC directly with spectroscopic methods, e.g., nuclear magnetic resonance (NMR) spectroscopy is an option. However, limited sensitivity of such methods deters their use. Today, mass spectrometry (MS) is the most suitable method of analyte characterization, due to its high sensitivity and the detailed mass and fragment information provided. Invention of soft ionization methods, such as matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) MS and electrospray ionization (ESI), made this possible; today, other desorption and atmospheric pressure ionization techniques are also available. This chapter covers the direct combination of TLC with MS, or TLC/ MS, and overviews different molecule classes that have been analyzed by this approach. The significant progress from 2005–2010 will be emphasized. Chromatographic Methods and Product Identification Planar chromatography (in particular thin-layer or high-performance thin-layer chromatography) and high-performance liquid chromatography (HPLC) are very important tools of modern analytical chemistry (Hahn- Deinstrop, 2006).

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Dr. Jurgen Schiller

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