Text Box: Nano-LIQUID CHROMATOGRAPHIC SEPARATIONS CHIARA FANALI1, MARÍA ASENSIO-RAMOS2, JAVIER HERNÁNDEZ-BORGES2, ANNA ROCCO3, AND SALVATORE FANALI3 1UNIVERSITY CAMPUS-BIOMEDICO, VIA ALVARO DEL PORTILLO, 21, 00128 ROME, ITALY; 2DEPARTAMENTO DE QUÍMICA ANALÍTICA, NUTRICIÓN Y BROMATOLOGÍA, FACULTAD DE QUÍMICA, UNIVERSIDAD DE LA LAGUNA (ULL), AVENIDA ASTROFÍSICO FRANCISCO SÁNCHEZ S/N, 38206 LA LAGUNA (TENERIFE), ESPAÑA; AND 3INSTITUTE OF CHEMICAL METHODOLOGIES, ITALIAN NATIONAL COUNCIL OF RESEARCH, AREA DELLA RICERCA DI ROMA I, VIA SALARIA KM 29,300, 00015 MONTEROTONDO SCALO, ROME, ITALY. Introduction Nano-liquid chromatography (nano-LC), first introduced by Karlsson and Novotny in 1988 (Karlsson & Novotny, 1988), is a miniaturized liquid chromatographic technique where the separation of analytes takes place on a capillary column containing selected stationary phases (SPs). Even though a standard definition of nano-LC has not been finalized, usually this name is assigned to those techniques employing capillaries of I.D. <100 μm and flow rates in the range 50–800 nL/min. In contrast, capillary-LC (CLC) makes use of 100–320 μm I.D. columns and flow rates in the range 1–100 μL/min. Since the boundary between nano-LC and CLC overlaps, both of them will be considered in this chapter. Furthermore, other techniques which employ capillaries, such as capillary zone electrophoresis (CZE) and Capillary Electrochromatography (CEC), will be discussed and compared due to the Similarity of their set up. In nano-LC, the relatively low flow-rate represents an interesting advantage over conventional techniques allowing the perfect coupling with mass spectrometry (MS). In addition: costs are reduced due to the limited use of mobile phases and consequently low waste makes this technique eco-friendly. In addition peak dilution during the chromatographic run is reduced with an increase of mass sensitivity. Finally high efficiency, good resolution and short analysis times are currently obtained (Karlsson & Novotny, 2988; Szumski & Buszewski, 2002; Hernandez-Borges et al., 2007a). Because of its features and advantages, nano-LC has been applied mainly in analytical chemistry for the analysis of a large number of compounds currently investigated in various fields such as proteomics, pharmaceutical, chiral, food and beverage, environmental samples, etc.

This was a sample page from the book to give you an idea of what is discussed. 

To learn more about Nano-Liquid Chromatography Separations, Buy the Book!

Contact the authors:

 

Prof. Dr. Chiara Fanali et al.

 

c/o AOCS Press

P.O. Box 17190

Urbana, IL 61803-7190

 

Phone: 217-359-2344

E-mail: sales@aocs.com

Web address:

http://www.aocs.org/Store/