Text Box: Preface ............................................................................................................................................................iv 1: The Theory and Practice of UHPLC and UHPLC-MS Davy Guillarme and Jean-Luc Veuthey ............................................................................................................1 2: Hydrophilic Interaction Chromatography—An Excellent Method for Separation of Polar Samples Pavel Jandera..................................................................................................................................................47 3: Chiral Separations Francesco Gasparrini, Ilaria D’Acquarica, Marco Pierini, and Claudio Villani ............................................. 103 4: High-temperature Liquid Chromatography Thorsten Teutenberg ................................................................................................................................... 129 5: High-efficiency Liquid Chromatography Separations Achieved by Monolithic Silica Columns Kosuke Miyamoto, Tohru Ikegami, Takeshi Hara, Yasushi Ishihama, and Nobuo Tanaka …..................... 175 6: Silver-ion High-performance Liquid Chromatography—Mass Spectrometry in the Analysis of Lipids Michal Holčapek and Miroslav Lisa ............................................................................................................. 197 7: Recent Advances in Comprehensive Two-dimensional Liquid Chromatography for the Analysis of Natural Products Francesco Cacciola, Paola Donato, Luigi Mondello, and Paola Dugo ........................................................ 231 8: Multiple Parallel Mass Spectrometry Techniques for Lipid and Vitamin D Analysis William Craig Byrdwell ................................................................................................................................ 255 9: Nano-liquid Chromatographic Separations Chiara Fanali, Maria Asensio-Ramos, Javier Hernandez-Borges, Anna Rocco, and Salvatore Fanali ...... 301 10: Comprehensive 2D GC Methodologies for the Analysis of Lipids Giorgia Purcaro, Peter Quinto Tranchida, and Luigi Mondello .................................................................... 381 . 11: Thin-layer Chromatography with Direct Mass Spectrometric Analyte Detection Beate Fuchs and Jurgen Schiller ................................................................................................................ 431