Figure 12-5. The influence of organic modifier type on ultra-high performance supercritical fluid chromatography (UHPSFC) separations. Chromatograms show separations of the active pharmaceutical ingredient (API) ticagrelor [blue (gray in print versions) 1] and its three impurities (2, 3, 4) on a Torus Diol column (100 mm x 3.0 mm, 1.7 um), with gradient elution 5%-40% of organic modifier in 3 min, flow-rate 1.5 mL/min, temperature 40oC, backpressure regulator 2000 psi, UV detection at 225 nm. ACN, acetonitrile; EtOH, ethanol; MeOH, methanol.
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