Handbook of Advanced Chromatography / Mass Spectrometry Techniques
Chapter 1 - Theory and Practice of UHPLC and UHPLC-MS
by Davy Guillarme and Jean-Luc Veuthey

Figure 1-2. Impact of particle size reduction on the Van Deemter curves. These experimental curves were obtained with a mobile phase: H2O/MeCN (60:40 v/v), with butylparaben 25 ppm in H2O, UV detection: 254 nm, columns: XTerra RP18 4.6 mm x 150 mm, 5 um; XTerra RP 18 4.6 mm x 50 mm, 3.5 um; Acquity Shield bridged ethylsiloxane/ silica hybrid C18 2.1 mm x 50 mm, 1.7 um.

Adapted from Nguyen, D.T.T., Guillarme D., Rudaz, S., Veuthey, J.L., 2006a. Fast analysis in liquid chromatography using small particles size and ultra-high pressure. J. Sep. Sci. 29, 1836-1848; Nguyen, D.T.T., Guillarme, D., Rudaz, S., Veuthey, J.L., 2006b. Chromatographic behaviour and comparison of column packed with sub-2 um stationary phases in liquid chromatography. J. Chromatogr. A 1128, 105-113, with permission.

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