Handbook of Advanced Chromatography / Mass Spectrometry Techniques
Chapter 1 - Theory and Practice of UHPLC and UHPLC-MS
by Davy Guillarme and Jean-Luc Veuthey

Figure 1-4. Illustration of the first ultrahigh-pressure liquid chromatography experiments made by the groups of Jorgenson et al. and Milton Lee et al. (A) Ultraefficient separation of small organic test compounds obtained; conditions: 7200 bar inlet pressure, capillary column (430 mm x 50 um I.D., packed with 1.0 um nonporous particles) (Jerkovich et al., 2003). (B) Ultrafast separation of a triazine herbicides mixture. Conditions: 2800 bar inlet pressure; capillary column (129 mm x 30 um I.D. packed with 1.5 um Kovasil mass spectrometry-H nonporous particles).

(B) Adapted from Lippert, J.A., Xin, B., Wu, N., Lee, M.L., 1999. Fast ultrahigh-pressure liquid chromatography: on-column UV and time-of-flight mass spectrometric detection. J. Microcolumn Sep. 11, 631-643, with permission.

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