Handbook of Advanced Chromatography / Mass Spectrometry Techniques
Chapter 1 - Theory and Practice of UHPLC and UHPLC-MS
by Davy Guillarme and Jean-Luc Veuthey

Figure 1-5. Performance comparison of liquid chromatography strategies in isocratic mode in terms of throughput (t0 for N = 10,000 plates) and maximal resolution (Nmax for t0 = 30 min) for a model compound, butylparaben with MW of 200 g/mol. The data were gathered using the kinetic plot methodology, considering a maximal pressure of 200 bar for monoliths, 400 bar for high-pressure liquid chromatography (HPLC), high-temperature liquid chromatography (HTLC) and sub-2 um, 600 bar for superficially porous particles (SPP) and 1000 bar for ultrahighpressure liquid chromatography (UHPLC) and HT-UHPLC.

Adapted from Guillarme, D., Ruta, J., Rudaz, S., Veuthey, J.-L., 2010a. New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches. Anal. Bioanal. Chem. 397, 1069-1082; Guillarme, D., Schappler, J., Rudaz, S., Veuthey, J.L., 2010b. Coupling ultrahigh pressure liquid chromatography with mass spectrometry. Trends Anal. Chem. 29, 15-27, with permission.

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