Figure 1-6. Ultrafast separations carried out in HT-ultrahigh-pressure liquid chromatography. (A) Isocratic separation of various preservatives and uracil. Column: Acquity bridged ethylsiloxane/ silica hybrid (BEH) C18 (50 x 2.1 mm I.D., 1.7 um); mobile phase: water-acetonitrile (50:50%, v/v); flow rate: 1800 uL/min; temperature: 90 ^oC; (B) Gradient separation of several doping agents. Column: Acquity BEH Shield RP18 (50 x 2.1 mm I.D., 1.7 um); mobile phase: 0.1% formic acid in water-0.1% formic acid in acetonitrile; flow rate: 1800 uL/min; temperature:
90 ^oC.

Adapted from Nguyen, D.T.T., Guillarme, D., Heinisch, S., Barrioulet, M.P., Rocca, J.L., Rudaz, S., Veuthey J.L., 2007. High throughput liquid chromatography with sub-2 um particles at very high pressure and high temperature. J. Chromatogr. A 1167, 76-84, with permission.

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