Handbook of Advanced Chromatography / Mass Spectrometry Techniques
Chapter 1 - Theory and Practice of UHPLC and UHPLC-MS
by Davy Guillarme and Jean-Luc Veuthey

Figure 1-7. Performance comparison of liquid chromatography strategies in gradient mode in terms of throughput (tgrad for P = 100) and maximal resolution (Pmax for tgrad = 3 h) for a model compound, butylparaben with MW of 200 g/mol. The data were gathered using the kinetic plot methodology applied to gradient elution and considering the same maximal pressure as for isocratic mode. HTLC, high-temperature liquid chromatography; HT-UHPLC, hightemperature ultrahigh-pressure liquid chromatography; SPP, superficially porous particles; UHPLC, ultrahighpressure liquid chromatography.

Adapted from Guillarme, D., Ruta, J., Rudaz, S., Veuthey, J.-L., 2010a. New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches. Anal. Bioanal. Chem. 397, 1069-1082; Guillarme, D., Schappler, J., Rudaz, S., Veuthey, J.L., 2010b. Coupling ultra-high pressure liquid chromatography with mass spectrometry. Trends Anal. Chem. 29, 15-27, with permission.

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