Handbook of Advanced Chromatography / Mass Spectrometry Techniques
Chapter 1 - Theory and Practice of UHPLC and UHPLC-MS
by Davy Guillarme and Jean-Luc Veuthey

Figure 1-8. 3D representation of the temperature profiles along and across various chromatographic columns (A) 150 mm x 2.1 mm (BEH) bridged ethylsiloxane/ silica hybrid-C18 column, flow rate = 0.95 mL/min, Pinlet = 979 bar. (B) 100 mm x 2.1 mm BEH-C18 column, flow rate = 1.45 mL/min, Pinlet = 1005 bar. (C) 50 mm x 2.1 mm BEH-C18 column, flow rate = 2 mL/min, Pinlet = 775 bar. The experimental setup used to measure the temperature of the surface of the column and the temperature of the liquid exiting the column outlet is also shown.

Adapted from Gritti, F., Guiochon, G., 2008. Complete temperature profiles in ultra-high-pressure liquid chromatography columns. Anal. Chem. 80, 5009-5020, with permission.

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