Figure 1-9. Isocratic method transfer from regular high-pressure liquid chromatography to ultrahigh-pressure liquid chromatography. Separation of a benzodiazepines mixture in isocratic mode with a mobile phase containing ACN-water (31:69, v/v) with 0.1% formic acid, T = 30^oC and wavelength = 254 nm. (A) Hypersil GOLD 150 x 4.6 mm, 5 um, F = 1000 uL/min, V_inj = 20 uL. (B) Hypersil GOLD 50 x 2.1 mm, 1.9 um, F = 550 uL/min, V_inj = 1.4 uL.

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