Figure 5-2. Fast reversed-phase separation of proteins. Conditions: Poly(styrene-co divinylbenzene) monolithic column 50 x 4.6 mm I.D., mobile phase gradient 42%-90% acetonitrile in 0.15% aqueous trifluoroacetic acid in 0.35 min, flow rate 10 mL/min, UV detection at 280 nm. Peaks: ribonuclease (1), cytochrome c (2), bovine serum albumin (3), carbonic anhydrase (4), and ovalbumin (5).

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