Figure 5-3. The separation of crude products from synthesis of triazole (m/z 176) using the mobile phase ethyl acetate-n-hexane (1:2 v/v) with 2% acetic acid and presented as ultrathin-layer chromatography (UTLC)-UV densitogram of the synthesis sample (top). Identification of peaks was achieved using atmospheric pressure matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry (MS) spectra of the by-product A (m/z 369) (center) and the product (m/z 198 [M+Na]+ and m/z 107) (bottom). The main matrix ions are marked with asterisks.
Adapted from Salo, P.K., Salomies, H., Harju, K., Ketola, R.A., Kotiaho, T., Yli-Kauhaluoma, J., Kostiainen, R., 2005. Analysis of small molecules by ultra thin-layer chromatography-atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry. J. Am. Soc. Mass Spectrom. 16, 906-915. Copyright American Society for Mass Spectrometry, 2005.
*Click on image for full-size .jpg*
<< Back to Chapter 5 <<