Figure 6-13. Lys-C peptide maps of trastuzumab obtained with (A) BEH C18 and (B) CSH C18. Peak widths at half height (w0.5) are shown for five peptides spread across the separations. Peak capacities (4s) were calculated from the averages of these values. (C) Corresponding retention windows from each peptide map. Colored lines indicate the change in elution order of the different peptide species, (peptides marked with blue lines (light gray in print versions) elute relatively earlier, peptides marked with red lines (gray in print versions) elute relatively later) as detected by electrospray ionization-mass spectrometry. The number of basic residues for each identified species is provided.

Reprinted with permission from Lauber, M.A., Koza, S.M., McCall, S.A., Alden, B.A., Iraneta, P.C., Fountain, K.J., 2013. Highresolution peptide mapping separations with MS-friendly mobile phases and charge-surface-modified C18. Anal. Chem. 85, 6936-6944.

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