Figure 7-12. Illustration of the dependence of effective two dimensional (2D) peak capacity (n_c,2D) on second dimension (2D) analysis time ²t_cycle. Calculations were made as follows: the native first dimension (¹D) analysis time and peak capacity (i.e., without undersampling) were assumed to be 30 and 100 min, respectively, and the effective ¹D peak capacity (A) was calculated using Eq. (7.5); the dependence of ²D peak capacity (B) on ²D cycle time is the same as that reported previously (Davis and Stoll, 2014); finally, the effective ²D peak capacity (C) is calculated as the product of the effective ¹D peak capacity and the ²D peak capacity at each value of ²t_cycle.

Adapted from Bedani, F., Schoenmakers, P.J., Janssen, H-G., 2012. Theories to support method development in comprehensive two-dimensional liquid chromatography - a review. J. Sep. Sci. 35, 1697-1711. http://dx.doi.org/10.1002/jssc.201200070.

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