Figure 7-13. Peak capacity as a function of analysis time for one dimensional liquid chromatography with different pressure limitations and for different analyte types. Blue (dark gray in print versions) circles are for small nonpeptide molecules with d(ln k)/d(f) = 8, and red (light gray in print versions) circles are for peptide molecules with d(ln k)/d(f) = 25. Open circles are for a pressure limit of 400 bar, and filled circles are for 1000 bar. Plate numbers are first estimated for dead times that are 10% of the analysis time assuming three-parameter optimization at 40oC (Carr et al., 2009), and then gradient peak capacity is calculated from these plate numbers using the approach prescribed by Neue (2005).

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