Handbook of Advanced Chromatography / Mass Spectrometry Techniques
Chapter 7 - Introduction to Two-Dimensional Liquid Chromatography - Theory and Practice
by Dwight R. Stoll

Figure 7-14. Numbers of peaks observed in separations of plant extracts. For LC x LC separations, the 2D cycle time was varied to demonstrate the optimum cycle timed2D cycle times are: 40 (open diamonds), 21, (open circles), 12 (open triangles), and 6 (open squares) sec. At analysis times of about 10 min, the two-dimensional liquid chromatography method outperforms the one dimensional liquid chromatography method (filled diamonds) and remains superior at all longer times.

Reprinted with permission from Huang, Y., Gu, H., Filgueira, M., Carr, P.W., 2011. An experimental study of sampling time effects on the resolving power of on-line two-dimensional high performance liquid chromatography. J. Chromatogr. A 1218, 2984-2994. http://dx.doi.org/10.1016/j.chroma.2011.03.032. Copyright 2011, Elsevier.

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