Handbook of Advanced Chromatography / Mass Spectrometry Techniques
Chapter 7 - Introduction to Two-Dimensional Liquid Chromatography - Theory and Practice
by Dwight R. Stoll

Figure 7-16. Conceptual comparison of LC-LC, LC x LC, and sLC x LC modes of two-dimensional liquid chromatography (2D-LC) separation. Panel A shows the case of LC-LC (heartcutting 2D-LC) where, in this case, only a single fraction is injected into the 2D column. Note that in multiple heartcutting (mLC-LC, not shown) more than one region of the first dimension (1D) effluent would be injected onto the second dimension (2D) column. Panel B shows the case of LC x LC (comprehensive 2D-LC) where the entire 1D effluent is sequentially injected. Panel C shows the case of sLC x LC (selective comprehensive 2D-LC) where selected segments of the 1D effluent are injected comprehensively on to the 2D column.

Reprinted with permission from Stoll, D., Carr, P., 2016. Two-dimensional liquid chromatography: a state-of-the art tutorial. Anal. Chem. (submitted for publication). http://dx.doi.org/10.1021/acs.analchem.6b03506. Copyright 2016, American Chemical Society.
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