Figure 7-20. Consequence of using a large sampling window [indicated by the red (dark gray in print versions) dashed vertical lines] in the case of single heartcut operation of two dimensional liquid chromatography. Sample components that had been separated from the target compound (red peak (dark gray in print versions)) by the 1D column are remixed and some are not reseparated (the teal peak (gray in print versions) here) from the target analyte by the 2D column.
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