Figure 7-32. Examples of dynamic elution profiles that can be used in the second dimension of two-dimensional liquid chromatography systems. In each plot the dashed trace represents the eluent composition used in the first dimension, and the solid trace represents the eluent composition used in the second dimension.

Reprinted with permission of Springer from Li, D., Schmitz, O.J., 2013. Use of shift gradient in the second dimension to improve the separation space in comprehensive two-dimensional liquid chromatography. Anal. Bioanal. Chem. 405, 6511-6517. http://dx.doi.org/10.1007/s00216-013-7089-5.

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