Handbook of Advanced Chromatography / Mass Spectrometry Techniques
Chapter 7 - Introduction to Two-Dimensional Liquid Chromatography - Theory and Practice
by Dwight R. Stoll

Figure 7-34. Illustration of the flow of information in a two-dimensional liquid chromatography experiment. The scenario shown here is precisely applicable to LC x LC, but can be extended to other implementations of 2D separation. (1) Peaks eluting from the 1D column are sampled multiple times, which leads to the appearance of a particular compound in multiple 2D chromatograms. (2) The data from these 2D separations are then transformed into a 2D array of data where the two dimensions are 1D and 2D time. This array can then be visualized as a contour plot or a 2D topographic map, both of which can be colored in various ways to aid visualization.

Adapted with permission from Adahchour, M., Beens, J., Vreuls, R.J.J., Brinkman, U.A.T., 2006. Recent developments in comprehensive two-dimensional gas chromatography (GCxGC) - introduction and instrumental setup. TrAC Trends Anal. Chem. 25, 438-454. http://dx.doi.org/10.1016/j.trac.2006.03.002. Copyright 2006, Elsevier.

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