Handbook of Advanced Chromatography / Mass Spectrometry Techniques
Chapter 7 - Introduction to Two-Dimensional Liquid Chromatography - Theory and Practice
by Dwight R. Stoll

Figure 7-9. Comparison of LC x LC separations of peptides using RP-LC in the first dimension and either RPeLC (A) or HILIC (B) separation in the second dimension. In spite of the higher fractional coverage in the RP x HILIC case, the effective 2D peak capacity is lower because of the low peak capacity of the HILIC 2D separation. HILIC, hydrophilic interaction liquid chromatography; LC, liquid chromatography; RP, reversed-phase.

Reprinted with permission from D’Attoma, A., Heinisch, S., 2013. On-line comprehensive two dimensional separations of charged compounds using reversed-phase high performance liquid chromatography and hydrophilic interaction chromatography. Part II: application to the separation of peptides. J. Chromatogr. A 1306, 27-36. http://dx.doi.org/10.1016/j.chroma.2013.07.048. Copyright 2013, Elsevier.

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