Figure 8-3. RP-LC x RP-LC contour plots of a sugarcane leaf extract by means of three different set-up, namely “full in fraction” (FIF, set-up 3A), “segmented in fraction” (SIF, set-up 3B), and “continuously shifting” (CS, set-up 3C) gradients. 1D, first dimension; RP-LC, reversed-phase liquid chromatography.

Reprinted with Permission from Springer: Leme, G.M., Cacciola, F., Donato, P., Cavalheiro, A.J., Dugo, P., Mondello, L., 2014. Continuous vs. segmented second-dimension system gradients for comprehensive two-dimensional liquid chromatography of sugarcane (Saccharum spp.). Anal. Bioanal. Chem. 406, 4315-4324.

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